SAKAGUCHI Masayoshi

School of Advanced Engineering Department of Chemistry and Life ScienceAssociate Professor

  • E-Mail: bt11532ns.kogakuin.ac.jp

    Career

    • Apr. 2017 - Present
      工学院大学先進工学部
    • Apr. 2015 - Mar. 2017
      工学院大学先進工学部
    • Apr. 2016 - Sep. 2016
      東京農工大学農学府農学部 非常勤講師「応用生物科学特別講義Ⅰ」
    • Apr. 2009 - Mar. 2015
      Kogakuin University, Faculty of Engineering, Department of Applied Chemistry
    • Apr. 1995 - Mar. 2009
      Kogakuin University, Faculty of Engineering, Department of Applied Chemistry

    Educational Background

    • Apr. 1993 - Mar. 1995
      Tokyo University of Agriculture and Technology, Graduate School, Division of Agriculture
    • Apr. 1989 - Mar. 1993
      Tokyo University of Agriculture and Technology, Faculty of Agriculture

    Degree

    • Mar. 1993
      学士(農学), Tokyo University of Agriculture and Technology
    • Mar. 1995
      修士(農学), Tokyo University of Agriculture and Technology
    • Sep. 2008
      Doctor of Philosophy in Engineering, Kogakuin University

    Affiliated academic society

    • Dec. 1997 - Present
      日本化学会
    • Mar. 1997 - Present
      日本農芸化学会
    • May 1995 - Present
      日本応用糖質科学会

    IDs

    • Identifiers

      研究者番号:80281351
      researchmap会員ID:1000195678
      J-Global ID:200901053258818762

    Research Field

    • Nanotechnology/Materials, Functional solid-state chemistry, Functional Material Chemistry
    • Nanotechnology/Materials, Biochemistry, Chemistry Related to Living Body
    • Life sciences, Structural biochemistry, Structural Biochemistry
    • Life sciences, Applied biochemistry, Applied Biochemistry

    Research Keyword

    • serine protease
    • chitinase
    • bacteria
    • archaea
    • glucoaymase
    • trehalase
    • GH15 family enzyme
    • carbohydrate-hydrolyzing enzyme
    • characterization
    • enzyme chemistry

    Research theme

    • 1998 - Present
      Analysis of function and structure in bacterial glucoamylase
      glucoamylse,function,strucuture
    • 1999 - 2011
      Analysis of relationship between function and structure for thermophilic serine protease
      thermophilic,serine protease,
    • 2002 - 2012
      Cloning, expression, and characterization of novel glucoamylase from Caulobacter crescentus CB15
      glucoamlyase,cloning,expression
    • 2011 - Present
      Characterization and protein engineering for Bacterial chitinases
      chitinase, chitin, chitin oligomer
    • 2011 - Present
    • 2011 - Present

    Books and other publications

    • Handbook of Proteolytic Enzymes, 3rd Edition
      2012
      9780123822192

    Paper

    MISC

    Lectures, oral presentations, etc.

    • 非昆虫ベースの食餌は,肉食性動物の酸性キチナーゼの構造と機能を変化させる
      17 Mar. 2022
    • ネコ酸性キチナーゼ偽遺伝子の活性化
      17 Mar. 2022
    • 好酸性アーケアThermoplasma volcanium GH15 トレハラーゼの立体構造解析
      16 Mar. 2022
    • ヒトとマウスキトトリオシダーゼの活性とアミノ酸配列比較
      26 Aug. 2021
    • 食肉目動物の食性と酸性キチナーゼの機能に関する研究
      26 Aug. 2021
    • キメラ体を利用したイヌの酸性キチナーゼの活性化
      26 Aug. 2021
    • ヒトとマウスキトトリオシダーゼのアミノ酸218位の置換による活性の評価
      21 Mar. 2021
    • GH15古細菌トレハラーゼの機能解析
      28 Mar. 2020
    • イヌとウシの酸性キチナーゼの不活性化
      27 Mar. 2020
    • Mouse chitinases have no synergistic effects on degradation of chitin
      Kimura; M.; Umeyama; T.; Wakita; S.; Okawa; K.; Sakaguchi; M. ; Oyama; F.
      ASHG 2019 annual meeting (Houston), 18 Oct. 2019, The American Society of Human Genetics (ASHG)
    • マウスにおけるジ-N-アセチルキトビアーゼの発現解析と酵素の性質解析
      19 Sep. 2019
    • FACE法によるマウスキチナーゼの相乗効果の決定
      29 Aug. 2019
    • マウスキチナーゼの相互作用に関する研究
      26 Mar. 2019
    • リステリアキチナーゼの機能未知領域の機能
      24 Mar. 2019
    • Quantification of chitinases mRNA levels by qPCR in crab-eating monkey tissues: Comparison with mouse and human.
      Uehara; M.; Tabata; E.; Ohno; M.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 2018, 68th Annual Meeting of ASHG (San Diego), 18 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Evaluations of enzymatic properties for human and mouse chitotriosidase
      Watanabe; T.; Kimura; M.; Wakita; S.; Sekine; K.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 2018, 68th Annual Meeting of ASHG (San Diego), 18 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Feeding behaviors determine acidic chitinase mRNA levels in mammalian and poultry stomachs
      Oyama; F.; Kashimura; A.; Kikuchi; A.; Masuda; H.; Miyahara; R.; Hiruma; Y.; Wakita; S.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Tabata; T.
      The American Society of Human Genetics (ASHG) 2018 68th Annual Meeting of ASHG (San Diego), 17 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Comparison of chitinolytic properties of mouse Chit1 and AMCase with Serratia marcescens chitinase B
      Kimura; M.; Umeyama; T.; Wakita; S.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 2018, 68th Annual Meeting of ASHG (San Diego), 17 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Chitinase 3-like-1 with amino acid substitutions at the active site remains inactive.
      Kishigami; N.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 2018, 68th Annual Meeting of ASHG (San Diego), 17 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Gene expression analysis of mammalian chitinases in common marmoset (<i>Callithrix jacchus) tissue
      Tabata; E.; Kashimura; A.; Uehara; M.; Wakita; S.; Sakaguchi; M.; Sugahara; Y.; Yurimoto; T.; Sasaki; E.; Oyama; F.
      The American Society of Human Genetics (ASHG) 2018, 68th Annual Meeting of ASHG (San Diego), 17 Nov. 2018, The American Society of Human Genetics (ASHG)
    • Acidic chitinase mRNA levels and their chitinolytic activity are affected by the feeding behavior, which may determine chitin digestibility in animals
      Tabata; E.; Kashimura; A.; Wakita; S.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 30 Aug. 2018, Japanese Society for Chitin and Chitosan
    • Functional differences between human and mouse chitotriosidase
      Watanabe; T.; Kimura; M.; Sekine; K.; Sakaguchi; M.; Oyama; F.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 29 Aug. 2018, Japanese Society for Chitin and Chitosan
    • Acidic mammalian chitinase is a major chitinase in stomach resistant to pepsin and trypsin/chymotrypsin digestion
      Oyama; F.; Kimura; M.; Okawa; K.; Onuki; R.; Nemoto; C.; Tabata; E.; Wakita; S.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Ohno; M.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 28 Aug. 2018, Japanese Society for Chitin and Chitosan
    • Comparison of chitinolytic activities among mouse mammalian chitinases and Serratia marcescens chitinase B
      Kimura; M.; Umeyama; T.; Wakita; S.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 28 Aug. 2018, Japanese Society for Chitin and Chitosan
    • Gene expression analysis of chitinases in crab-eating monkey: species-specific expression of acidic mammalian chitinase and chitotriosidase
      Uehara; M.; Tabata; E.; Ohno; M.; Sakaguchi; M.; Oyama; F.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 28 Aug. 2018, Japanese Society for Chitin and Chitosan
    • Amino acid substitutions at the active site of Chitinase 3-like-1
      Kishigami; N.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      14th International Chitin and Chitosan Conference (14th ICCC) & ,12th Asia-Pacific Chitin and Chitosan Symposium (12th APCCS) ,(Joint with 32nd Japanese Society for Chitin and Chitosan Conference (32nd JSCCC)), 28 Aug. 2018, Japanese Society for Chitin and Chitosan
    • 2種類のマウスほ乳類キチナーゼのキチナーゼ活性比較
      17 Mar. 2018
    • ヒト酸性ほ乳類キチナーゼの nonsynonymous SNPs による活性の調節
      17 Mar. 2018
    • ニワトリ酸性キチナーゼの消化酵素としての機能解析
      17 Mar. 2018
    • 古細菌トレハラーゼのN末端領域の機能
      16 Mar. 2018
    • Chicken acidic chitinase can function as digestive enzyme which degrades chitin-containing organisms under its gastrointestinal tract
      Tabata; E.; Kashimura; A.; Wakita; S.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 16th International Symposium on Advanced Technology (ISAT-16), 02 Nov. 2017, Kogakuin University
    • Comparative analysis of mouse mammalian chitinases and Serratia marcescens chitinase B
      Kimura; M.; Umeyama; T.; Wakita; S.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      The 16th International Symposium on Advanced Technology (ISAT-16), 02 Nov. 2017, Kogakuin University
    • Characterization of Escherichia coli-produced mouse chitotriosidase and human chitotriosidase
      Watanabe; T.; Kimura; M.; Sekine; K.; Sakaguchi; M.; Oyama; F.
      The 16th International Symposium on Advanced Technology (ISAT-16), 02 Nov. 2017, Kogakuin University
    • Enzymatic properties of acidic mammalian chitinase under somatic tissue pH conditions
      Wakita; S.; Kimura; M.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 16th International Symposium on Advanced Technology (ISAT-16), 02 Nov. 2017, Kogakuin University
    • Gene expression analysis of chitinases by qPCR in crab-eating monkey tissues
      Uehara; M.; Tabata; E.; Ohno; M.; Sakaguchi; M.; Oyama; F.
      The 16th International Symposium on Advanced Technology (ISAT-16), 02 Nov. 2017, Kogakuin University
    • Gene expression analysis and enzyme function of pig mammalian chitinase
      Tabata; E.; Kashimura; A.; Wakita; S.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The American Society of Human Genetics (ASHG) 67th Annual Meeting (Orlando), 20 Oct. 2017, The American Society of Human Genetics (ASHG)
    • Comparison of chitinolytic activities among mammalian chitinases and bacterial chitinase
      Kimura; M.; Umeyama; T.; Wakita; S.; Okawa; K.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 67th Annual Meeting (Orlando), 19 Oct. 2017, The American Society of Human Genetics (ASHG)
    • Mechanistic insight of inactivation of mouse chitinase-like protein Ym1
      Oyama; F.; Sakaguchi; M.; Sugahara; Y.; Okawa; K.
      The American Society of Human Genetics (ASHG) 67th Annual Meeting (Orlando), 19 Oct. 2017, The American Society of Human Genetics (ASHG)
    • Chitinases mRNA levels quantified by qPCR in crab-eating monkey tissues.
      Uehara; M.; Tabata; E.; Ohno; M.; Sakaguchi; M.; Oyama; F.
      The American Society of Human Genetics (ASHG) 67th Annual Meeting (Orlando), 18 Oct. 2017, The American Society of Human Genetics (ASHG)
    • 古細菌トレハラーゼのN末端領域の役割
      07 Sep. 2017
    • 新規古細菌トレハラーゼの大腸菌組換えタンパク質の発現及び性質解析
      07 Sep. 2017
    • ヒト酸性ほ乳類キチナーゼの進化学的解析
      24 Aug. 2017
    • キチンオリゴ糖蛍光標識法を用いた酸性ほ乳類キチナーゼの特性解析
      23 Aug. 2017
    • カニクイザルにおけるキチナーゼ遺伝子の発現解析
      23 Aug. 2017
    • ニワトリ酸性キチナーゼの遺伝子発現解析とその酵素機能
      23 Aug. 2017
    • マウスほ乳類キチナーゼの比較解析
      23 Aug. 2017
    • ニワトリ組織におけるキチナーゼ遺伝子発現レベルとその酵素学的機能の解析
      18 Mar. 2017
    • Functional analysis of acidic mammalian chitinase under physiological conditions
      Wakita; S.; Kimura; M.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 21 Oct. 2016, the American Society of Human Genetics (ASHG)
    • Loss and recovery of chitinolytic activity by amino acid substitutions in human acidic mammalian chitinase
      Okawa; K.; Ohno; M.; Kashimura; A.; Kobayashi; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 21 Oct. 2016, the American Society of Human Genetics (ASHG)
    • Protein A-mouse Chit1-V5-His expressed in <i>Escherichia coli possesses chitinase functions comparable to CHO-expressed protein
      Kimura; M.; Wakita; S.; Ishikawa; K. Sekine; K. Yoshikawa; S.; Sato; A. Okawa; K.; Kashimura; A.; Yamanaka; D.; Ohno; N.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 20 Oct. 2016, the American Society of Human Genetics (ASHG)
    • Gene expression analysis of mammalian chitinase transcripts in pig tissues
      Tabata; E.; Ohno; M.; Sakaguchi; M.; Oyama; F.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 20 Oct. 2016, the American Society of Human Genetics (ASHG)
    • Inactivation of mouse AMCase by introducing amino acid substitutions encoded by single-nucleotide polymorphisms in human AMCase gene
      Oyama; F.; Okawa; K.; Ohno; M.; Kashimura; A. Kobayashi; Y. Sakaguchi; M.; Sugahara; Y.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 19 Oct. 2016, the American Society of Human Genetics (ASHG)
    • Analysis of chitinases from nonpathogenic bacterium Listeria innocua
      Honda; S.; Wakita; S.; Sugahara; Y.; Oyama; F.; Sakaguchi; M.
      the American Society of Human Genetics (ASHG) 66th Annual Meeting (Vancouver), 19 Oct. 2016, the American Society of Human Genetics (ASHG)
    • マウス胃における酸性ほ乳類キチナーゼの発現と生産細胞の同定
      19 Aug. 2016
    • ヒトとマウスの酸性哺乳類キチナーゼの機能解析と比較
      19 Aug. 2016
    • マウスキトトリオシダーゼは生理的条件下でキチン基質を分解する
      18 Aug. 2016
    • C末端欠損 Listeria innocuaキチナーゼの性質
      18 Aug. 2016
    • ブタ組織におけるキチナーゼ遺伝子発現レベルの解析
      18 Aug. 2016
    • AMCase による酸性~中性条件キチン分解産物の解析
      18 Aug. 2016
    • 細菌グルコアミラーゼのN末端配列の役割
      29 Mar. 2016
    • マウスの胃における酸性ほ乳類キチナーゼの発現と活性
      29 Mar. 2016
    • キメラ体を利用したヒトとマウスAMCaseの機能比較
      29 Mar. 2016
    • マウスキトトリオシダーゼの大腸菌のペリプラズムでの発現
      28 Mar. 2016
    • Comparison of degradation products from various substrates by Listeria chitinases
      Honda; S.; Sugahara; Y.; Oyama; F.; Sakaguchi; M.
      The 14th International Symposium on Advanced Technology(ISAT-14), 02 Nov. 2015, Kogakuin University
    • Characterization of Escherichia coli-produced mouse chitotriosidase
      Kimura; M.; Wakita; S.; Ishikawa; K.; Sekine; K.; Yoshikawa; S.; Okawa; K.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 14th International Symposium on Advanced Technology(ISAT-14), 02 Nov. 2015, Kogakuin University
    • Expression and characterization of catalytic domain of mouse AMCase in Escherichia coli.
      Oyama; F.; Kashimura; A.; Kimura; M.; Okawa; K.; Suzuki; H.; Ukita; A.; Wakita; S.; Okazaki; K.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.
      American Society of Human Genetics 65th Annual Meeting (Baltimore), 09 Oct. 2015, American Society of Human Genetics (ASHG)
    • Gene expression analysis of chitinase-like protein, YKL-40, with mammalian chitinases using qPCR in normal human tissues.
      Ohno; M.; Kida; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics 65th Annual Meeting (Baltimore), 09 Oct. 2015, American Society of Human Genetics (ASHG)
    • Enzymatic properties of mouse chitotriosidase expressed in Escherichia coli.
      Kimura; M.; Ishikawa; K.; Sekine; K.; Yoshikawa; S.; Wakita; S. Sato; A.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics 65th Annual Meeting (Baltimore), 08 Oct. 2015, American Society of Human Genetics (ASHG)
    • Biochemical analysis of chimeric human and mouse AMCase proteins expressed in Escherichia coli.
      Okawa; K.; Ohno; M.; kashimura; A.; Kobayashi; Y.; Sakaguchi; M.; Sugahara; Y.; Fumitaka; F.
      American Society of Human Genetics 65th Annual Meeting (Baltimore), 08 Oct. 2015, American Society of Human Genetics (ASHG)
    • 哺乳類キチナーゼとキチナーゼ様タンパク質の遺伝子発現解析
      20 Aug. 2015
    • 大腸菌で発現したマウス酸性哺乳類キチナーゼの触媒ドメインの性質
      20 Aug. 2015
    • 二成分モノマーを用いたキトサングラフト体(CS-g-PAM-PAA)の凝集特性
      20 Aug. 2015
    • Fluorophoreを用いたキチンオリゴ糖蛍光標識法の改良
      20 Aug. 2015
    • ヒトとマウス酸性哺乳類キチナーゼのキメラ体の作製とその解析
      20 Aug. 2015
    • マウスキトトリオシダーゼの大腸菌での発現とその性質
      20 Aug. 2015
    • Listeria属キチナーゼの分解産物比較
      20 Aug. 2015
    • Listeria由来キチナーゼの機能解析
      29 Mar. 2015
    • マウス組織におけるキチナーゼ様タンパク質の遺伝子発現解析
      28 Mar. 2015
    • マウス酸性哺乳類キチナーゼの触媒ドメインの大腸菌での発現とその性質
      28 Mar. 2015
    • 古細菌トレハラーゼの発現と諸性質の解析
      27 Mar. 2015
    • Expression of Listeria innocua chitinases in Escherichia coli and comparison of their properties
      Honda; S.; Sugahara; Y.; Oyama; F.; Sakaguchi; M.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 02 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Expression of mouse chitotoriosidase in Escherichia coli
      Kimura; M.; Ishikawa; K.; Sekine; K.; Yoshikawa; S.; Sato; A.; Okawa; K.; Kashimura; A.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 02 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Comparison of mRNA levels among Chit1, AMCase and reference genes in mouse tissues
      Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 02 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Characterization of Escherichia coli-produced human acidic mammalian chitinase
      Okawa; K.; Kashimura; A.; Kobayashi; Y.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 02 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Expression of mouse acidic mammalian chitinase in periplasmic space of Escherichia coli
      Kashimura; A.; Okawa; K.; Ishikawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 02 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Biochemical analysis of mammalian chitinases
      Oyama; F.; Ohno; M.; Kashimura; A.; Okawa; K.; Ishikawa; K.; Sakaguchi; M.; Sugahara; Y.
      The 1st Innovation Forum of Advanced Engineering Education (IFAEE)/The 21st International SPACC symposium (SPACC), 01 Nov. 2014, Kogakuin University and The Society of Pure and Applied Coordination Chemistry
    • Cross-species gene expression analysis of chitinase-like proteins with mammalian chitinases using qPCR in mouse and human tissues.
      Oyama; F.; Ohno; M.; Kida; Y.; Sakaguchi; M.; Sugahara; Y.
      64th Annual Meeting of the American Society of Human Genetics (San Diego), 20 Oct. 2014, American Society of Human Genetics
    • Expression of human acidic mammalian chitinase in Escherichia coli and analysis of its properties.
      Okawa; K.; Kashimura; A.; Kobayashi; Y.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      64th Annual Meeting of the American Society of Human Genetics (San Diego), 20 Oct. 2014, American Society of Human Genetics
    • Enzymatic properties of the catalytic domain of mouse acidic mammalian chitinase expressed in Escherichia coli.
      Kashimura; A.; Okawa; K.; Kimura; M.; Okazaki; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      64th Annual Meeting of the American Society of Human Genetics (San Diego), 20 Oct. 2014, American Society of Human Genetics
    • Analysis of the expression levels of chitinase-like proteins, Ym1, Ym2 and breast regression protein-39, in mouse tissues.
      Ohno; M.; Kida; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      64th Annual Meeting of the American Society of Human Genetics (San Diego), 19 Oct. 2014, American Society of Human Genetics
    • 細菌グルコアミラーゼのドメインの役割
      24 Sep. 2014
    • 哺乳類キチナーゼの遺伝子発現解析と大腸菌での発現
      07 Aug. 2014
    • 酸性哺乳類キチナーゼのpH依存的キチン分解産物の検討
      07 Aug. 2014
    • Acidic Mammalian Chitinaseの触媒ドメインの大腸菌での発現
      07 Aug. 2014
    • マウス組織でのキチナーゼとキチナーゼ様タンパク質の発現レベルの解析
      07 Aug. 2014
    • キトサンへの二成分モノマーのグラフト重合
      07 Aug. 2014
    • Expression in Escherichia coli and characterization of two chitinases from Listeria innocua
      ○S. Honda; Y. Sugahara; F. Oyama; M. Sakaguchi
      30 Mar. 2014
    • Characterization of Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of Escherichia coli
      ○K. Okawa; A. Kashimura; K. Ishikawa; Y. Kida; K. Iwabuchi; Y. Matsushima; M. Sakaguchi; Y. Sugahara; F. Oyama
      29 Mar. 2014
    • Analysis of mammalian chitinase mRNA levels in human and mouse tissues
      ○M. Ohno; Y. Togashi; K. Tsuda; K. Okawa; M. Kamaya; M. Sakaguchi; Y. Sugahara; F. Oyama
      28 Mar. 2014
    • Cloning and expression in Escherichia coli and functional identification of Caulobacter glucoamylase
      M. Sakaguchi; Y. Matsushima; T. Nankumo; J. Seino; S. Miyakawa; S. Honda; Y. Sugahara; F. Oyama; M. Kawakita
      28 Mar. 2014
    • Caulobacter グルコアミラーゼの発現と諸性質の解析
      2014
    • マウスとヒト組織における哺乳類キチナーゼ mRNA レベルの解析
      2014
    • Listeria属由来キチナーゼの発現と性質の解析
      2014
    • Cloning and expression in <i>Escherichia coli and functional identification of <i>Caulobacter glucoamylase
      2014
    • Characterization of Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of <i>Escherichia coli
      2014
    • Expression in <i>Escherichia coli and characterization of two chitinases from <i>Listeria innocua
      2014
    • 大腸菌のペリプラズム空間で発現させた酸性哺乳類キチナーゼは CHO 細胞で発現した組換え体と同等の機能を有する
      14 Dec. 2013
    • マウスとヒトにおける哺乳類キチナーゼの発現レベルの解析
      14 Dec. 2013
    • Characterization of Escherichia coli-expressed acidic mammalian chitinase
      Kashimura; A.; Okawa; K.; Ishikawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 25 Oct. 2013, American Society of Human Genetics (ASHG)
    • Quantitative expression analysis of mammalian chitinases in human and mouse tissues
      Oyama; F.; Ohno; M.; Togashi; Y.; Tsuda; K.; Okawa; K.; Sakaguchi; M.; Sugahara; Y.
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 24 Oct. 2013, American Society of Human Genetics (ASHG)
    • Species-specific expression of acidic mammalian chitinase in stomach tissues
      Ohno; M.; Togashi; Y.; Tsuda; K.; Okawa; K.; Kamaya; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 23 Oct. 2013, American Society of Human Genetics (ASHG)
    • Small introns of firefly luciferases: structural characterization and their insufficient substrates for splicing in CHO cells
      Ishii; M.; Kojima; R.; Fukuda; S.; Tanji; Y.; Sakaguchi; M.; Sugahara; Y.; Kamaya; M.; Oyama; F.
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 23 Oct. 2013, American Society of Human Genetics (ASHG)
    • Expression of mouse acidic mammalian chitinase in periplasmic space of Escherichia coli
      Okawa; K.; Kashimura; A.; Ishikawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 23 Oct. 2013, American Society of Human Genetics (ASHG)
    • Quantification of chitinase mRNA levels in mouse tissues by real-time PCR: acidic mammalian chitinase is a major transcript in the mouse stomach
      Oyama; F.; Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.
      10th Asia-Pacific chitin & chitosan symposium, 06 Oct. 2013
    • Expression in Escherichia coli and characterization of two chitinases from Listeria innocua
      Honda; S.; Sugahara; Y.; Oyama; F.; Sakaguchi; M.
      10th Asia-Pacific chitin & chitosan symposium, 06 Oct. 2013
    • Expression of mouse acidic mammalian chitinase in the periplasmic space fraction of Escherichia coli
      Okawa; K.; Kashimura; A.; Ishikawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      10th Asia-Pacific chitin & chitosan symposium, 06 Oct. 2013
    • Mouse acidic mammalian chitinase expressed in Escherichia coli possesses chitinase functions comparable to CHO-expressed protein
      Ishikawa; K.; Kashimura; A.; Okawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      10th Asia-Pacific chitin & chitosan symposium, 06 Oct. 2013
    • Preparation of chitin oligomers by hydrolysis with concentrated hydrochloric acid
      Sugahara; Y.; Kazami; N.; Sakaguchi; M.; Oyama; F. Kawakita; M.
      10th Asia-Pacific chitin & chitosan symposium, 05 Oct. 2013
    • Quantitative PCR comparison of mRNA levels in human and mouse
      Ohno; M.; Togashi; Y. Tsuda; K. Okawa; K.; Kamaya; M. Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      10th Asia-Pacific chitin & chitosan symposium, 05 Oct. 2013
    • Characterization of Escherichia coli-expressed mouse acidic mammalian chitinase
      Kashimura; A.; Okawa; K.; Ishikawa; K.; Kida; Y.; Iwabuchi; K.; Matsushima; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      10th Asia-Pacific chitin & chitosan symposium, 05 Oct. 2013
    • 好冷性グルコアミラーゼの大腸菌による発現と諸性質
      25 Sep. 2013
    • Preparation of chitin oligomers by hydrolysis with concentrated hydrochloric acid
      2013
    • Quantitative PCR comparison of mRNA levels in human and mouse
      2013
    • Characterization of Escherichia coli-expressed mouse acidic mammalian chitinase
      2013
    • Quantification of chitinase mRNA levels in mouse tissues by real-time PCR: acidic mammalian chitinase is a major transcript in the mouse stomach
      2013
    • Expression in Escherichia coli and characterization of two chitinases from Listeria innocua
      2013
    • Expression of mouse acidic mammalian chitinase in the periplasmic space fraction of Escherichia coli
      2013
    • Mouse acidic mammalian chitinase expressed in Escherichia coli possesses chitinase functions comparable to CHO-expressed protein
      2013
    • Species-specific expression of acidic mammalian chitinase in stomach tissues
      2013
    • Expression of mouse acidic mammalian chitinase in periplasmic space of Escherichia coli
      2013
    • Small introns of firefly luciferases: structural characterization and their insufficient substrates for splicing in CHO cells
      2013
    • Quantitative expression analysis of mammalian chitinases in human and mouse tissues
      2013
    • Characterization of Escherichia coli-expressed acidic mammalian chitinase
      2013
    • Characterization of Escherichia coli-expressed mouse acidic mammalian chitinase
      10th Asia-Pacific chitin & chitosan symposium, 2013
    • Expression in Escherichia coli and characterization of two chitinases from Listeria innocua
      10th Asia-Pacific chitin & chitosan symposium, 2013
    • Expression of mouse acidic mammalian chitinase in the periplasmic space fraction of Escherichia coli
      10th Asia-Pacific chitin & chitosan symposium, 2013
    • Mouse acidic mammalian chitinase expressed in Escherichia coli possesses chitinase functions comparable to CHO-expressed protein
      10th Asia-Pacific chitin & chitosan symposium, 2013
    • Expression of mouse acidic mammalian chitinase in periplasmic space of Escherichia coli
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 2013
    • Characterization of Escherichia coli-expressed acidic mammalian chitinase
      American Society of Human Genetics (ASHG) Annual meeting 2013 (Boston), 2013
    • Functional analysis of mouse chitinase-like protein Ym1
      Kida; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) 62nd Annual meeting (San Francisco), 09 Nov. 2012, American Society of Human Genetics (ASHG)
    • The Japanese firefly, Luciola cruciata, gene: Small intron and their insufficiency of splicing in CHO cells
      Ishii; M.; Kojima; R.; Fukuda; S.; Sakaguchi; M.; Sugahara; Y.; Kamaya; M.; Oyama; F.
      American Society of Human Genetics (ASHG) 62nd Annual meeting(San Francisco), 09 Nov. 2012, American Society of Human Genetics (ASHG)
    • Quantification of chitinase transcripts in human tissues.
      Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) 62nd Annual meeting (San Francisco), 07 Nov. 2012, American Society of Human Genetics(ASHG)
    • Quantitative expression analysis of mouse chitinases: Acidic mammalian chitinase is the major transcript in stomach
      Oyama; F.; Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.
      American Society of Human Genetics (ASHG) 62nd Annual meeting (San Francisco), 07 Nov. 2012, American Society of Human Genetics (ASHG)
    • Expression of mouse acidic mammalian chitinase in Escherichia coli and characterization of its properties.
      Kashimura; A.; Kida; Y.; Ohno; M.; Ishikawa; K.; Sakaguchi; M.; Oyama; F.
      American Society of Human Genetics (ASHG) 62nd Annual meeting (San Francisco), 07 Nov. 2012, American Society of Human Genetics (ASHG)
    • Utilization and availability of chitosan phthalate derivative for oral delivery
      Suzuki; S.; Sakaguchi; M.; Oyama; F.; Sugahara; Y.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • Functional comparison of recombinant chitinase-like protein Ym1 with AMCase
      Kida; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • The analysis of level of chitinases gene expression in mouse tissues
      Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • Characterization of recombinant mouse acidic mammalian chitinase
      Kashimura; A.; Ohno; M.; Ishikawa; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • A simple procedure for preparing chitin oligomers
      Sugahara; Y.; Kazami; N.; Sakaguchi; M.; Oyama; F.; Kawakita; M.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • Gene expression analysis of chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) in mouse tissues
      Oyama; F.; Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • Characterization of Listeria innocua chitinase expressed in Escherichia coli
      Honda; S.; Sugahara; Y.; Oyama; F.; Sakaguchi; M.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • Small Introns of the Japanese firefly, Luciola cruciata, and their insufficient substrates for splicing in mammalian cells
      Ishii; M.; Kojima; R.; Fukuda; S.; Sakaguchi; M.; Sugahara; Y.; Kamaya; M.; Oyama; F.
      The 11th International Symposium on Advanced Technology(ISAT-special), 30 Oct. 2012, Kogakuin University
    • 哺乳類キチナーゼのマウスとヒトでの発現制御
      13 Jul. 2012
    • Graft copolymerization of acrylamide onto chitosan
      13 Jul. 2012
    • Production of acidic mammalian chitinase in <i>Escherichia coli and characterization of its properties
      12 Jul. 2012
    • Expression of human AMCase variants in <i>Escherichia coli and analysis of their properties
      12 Jul. 2012
    • Quantification of the levels of chitinase transcripts in mouse tissues
      12 Jul. 2012
    • Application of N-carboxyacylchitosan for drug delivery system
      12 Jul. 2012
    • Expression of <i>Listeria chitinases in Escherichia coli and characterization of their properties
      12 Jul. 2012
    • 好熱性タンパク質分解酵素の熱安定に関わるイオン結合の役割
      25 Mar. 2012
    • 組換えマウス酸性キチナーゼの発現とその性質
      25 Mar. 2012
    • マウス組織におけるキチナーゼ遺伝子発現レベルの解析
      2012
    • 細菌Listeria属由来キチナーゼの発現と性質の解析
      2012
    • N-カルボキシアシルキトサンのDDSへの応用
      2012
    • キトサンへのアクリルアミドのグラフト重合
      2012
    • Utilization and availability of chitosan phthalate derivative for oral delivery
      2012
    • Characterization of Listeria innocua chitinase expressed in Escherichia coli
      2012
    • Functional comparison of recombinant chitinase-like protein Ym1 with AMCase
      2012
    • The analysis of level of chitinases gene expression in mouse tissues
      2012
    • Characterization of recombinant mouse acidic mammalian chitinase
      2012
    • Small Introns of the Japanese firefly, Luciola cruciata, and their insufficient substrates for splicing in mammalian cells
      2012
    • A simple procedure for preparing chitin oligomers
      2012
    • Gene expression analysis of chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) in mouse tissues
      2012
    • Quantification of chitinase transcripts in human tissues.
      2012
    • Quantitative expression analysis of mouse chitinases: Acidic mammalian chitinase is the major transcript in stomach
      2012
    • Expression of mouse acidic mammalian chitinase in Escherichia coli and characterization of its properties.
      2012
    • The Japanese firefly, Luciola cruciata, gene: Small intron and their insufficiency of splicing in CHO cells
      2012
    • Functional analysis of mouse chitinase-like protein Ym1
      2012
    • Production of acidic mammalian chitinase in Escherichia coli and characterization of its properties
      2012
    • Expression of human AMCase variants in Escherichia coli and analysis of their properties
      2012
    • Expression of Listeria chitinases in Escherichia coli and characterization of their properties
      2012
    • Characterization of Listeria innocua chitinase expressed in Escherichia coli
      The 11th International Symposium on Advanced Technology(ISAT-special), 2012
    • Small Introns of the Japanese firefly, Luciola cruciata, and their insufficient substrates for splicing in mammalian cells
      The 11th International Symposium on Advanced Technology(ISAT-special), 2012
    • Expression of mouse acidic mammalian chitinase in Escherichia coli and characterization of its properties.
      American Society of Human Genetics (ASHG) 62nd Annual meeting (San Francisco), 2012
    • The Japanese firefly, Luciola cruciata, gene: Small intron and their insufficiency of splicing in CHO cells
      American Society of Human Genetics (ASHG) 62nd Annual meeting(San Francisco), 2012
    • マウス組織におけるキチナーゼ遺伝子発現レベルの解析
      12 Oct. 2011
    • Quantification of chitinase transcripts in mouse
      Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) 61st Annual meeting (Montreal), 12 Oct. 2011, The American Society of Human Genetics
    • Regulation fo gene expression of mammalian chitinases
      Oyama; F.; Ohno; M.; Tsuda; K.; Sakaguchi; M.; Sugahara; Y.
      American Society of Human Genetics (ASHG) 61st Annual meeting (Montreal), 12 Oct. 2011, The American Society of Human Genetics
    • Expression of mouse chitinase in Escherichia coli and characterization of its properties
      Kashimura; A.; Ishikawa; K.; Sekine; K.; Kida; Y.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      American Society of Human Genetics (ASHG) 61st Annual meeting (Montreal), 12 Oct. 2011, The American Society of Human Genetics
    • N-カルボキシアシルキトサンの合成とその薬物送達システムへの応用
      31 Aug. 2011
    • 哺乳類キチナーゼの遺伝子発現制御
      31 Aug. 2011
    • マウスキチナーゼの大腸菌での発現とその性質
      30 Aug. 2011
    • マウス組織におけるキチナーゼ遺伝子発現レベルの解析
      2011
    • Quantification of chitinase transcripts in mouse
      2011
    • Regulation fo gene expression of mammalian chitinases
      2011
    • Expression of mouse chitinase in Escherichia coli and characterization of its properties
      2011
    • Expression of mouse chitinase in Escherichia coli and characterization of its properties
      American Society of Human Genetics (ASHG) 61st Annual meeting (Montreal), 2011
    • Role of ion pairs on stability and activity in a thermophilic serine protease
      Ohsaku; K.; Sakaguchi; M.; Sugahara; Y.; Kawakita; M.; Oyama; F.
      2010 International Chemical Congress of Pacific Basin Societies, 17 Dec. 2010
    • Cloning, expression and characterization of psychrophilic glucoamylase from Caulobacter crescentus CB15
      Sakaguchi; M.; Nankumo; T.; Seino; J.; Miyakawa; S.; Harakawa; H.; Yamamoto; M.; Sugahara; Y.; Oyama; F.; Kawakita; M.
      2010 International Chemical Congress of Pacific Basin Societies, 17 Dec. 2010
    • Regulation of expression of chitinase genes in mouse tissues
      Oyama; F.; Tsuda; K.; Ohno; M.; Sakaguchi; M.; Sugahara; Y.
      The American Society of Human Genetics (ASHG) 60th annual meeting (Washington), 05 Nov. 2010, The American Society of Human Genetics
    • Expression of chitin binding domain of mouse chitinase in Escherichia coli and characterization of its properties
      Kashimura; A.; Kojima; M.; Sakaguchi; M.; Sugahara; Y.; Oyama; F.
      The American Society of Human Genetics (ASHG) 60th annual meeting (Washington), 05 Nov. 2010, The American Society of Human Genetics
    • マウスにおけるキチナーゼの遺伝子発現制御
      14 Jul. 2010
    • キチンオリゴマーの生成条件の検討
      13 Jul. 2010
    • マウスキチナーゼのキチン結合ドメインの大腸菌での発現とその性質
      13 Jul. 2010
    • Expression of chitin binding domain of mouse chitinase in Escherichia coli and characterization of its properties
      2010
    • Regulation of expression of chitinase genes in mouse tissues
      2010
    • 好熱性セリンプロテアーゼの活性と安定性におけるイオン結合の役割
      2010
    • Cloning, expression and characterization of psychrophilic glucoamylase from Caulobacter crescentus CB15
      2010
    • Expression of chitin binding domain of mouse chitinase in Escherichia coli and characterization of its properties
      The American Society of Human Genetics (ASHG) 60th annual meeting (Washington), 2010
    • Cloning, expression and characterization of psychrophilic glucoamylase from Caulobacter crescentus CB15
      2010 International Chemical Congress of Pacific Basin Societies, 2010
    • キトサングラフト体の合成と特性解析
      21 Aug. 2009
    • キチン加水分解物の分画
      06 Aug. 2008
    • キトサングラフトポリアクリル酸の重金属吸着能
      06 Aug. 2008
    • 硫酸化キチンへのメタクリル酸メチルのグラフト重合
      27 Jul. 2007
    • 細菌<i>Caulobacter crescentus CB15のグルコアミラーゼ様遺伝子の発現とその解析
      27 Sep. 2006
    • N12-Diacetylspermine in the urine of cancer patients
      Hiramatsu; K.; Takahashi; K.; Kawaguchi; M.; Aiba; Y.; Sakaguchi; M.; Tamamori; Y.; Yamaguchi; T.; Mori; T.; Kawakita; M.
      International Conference on the Role of Polyamines and their Analogs in Cancer and other Diseases, 13 Sep. 2006
    • ヒトCMP-シアル酸輸送体の基質特異性決定領域
      25 Aug. 2006
    • 酸加水分解によるキチンオリゴマーの調製
      10 Aug. 2006
    • 細菌Caulobacter crescentus CB15のグルコアミラーゼ様遺伝子の発現とその解析
      2006
    • キチングラフトポリアクリロニトリルの合成とその加水分解反応
      05 Aug. 2005
    • 二段階加水分解によるキチンオリゴ糖の調製
      05 Aug. 2005
    • アシル化キトサン誘導体の合成とたんぱく包括能
      05 Aug. 2005
    • キトサンの塩酸加水分解挙動
      04 Aug. 2005
    • 細菌グルコアミラーゼの保存領域内アミノ酸の機能解析
      29 Mar. 2005
    • <i>Caulobacter crescentus CB15由来グルコアミラーゼの解析
      29 Mar. 2005
    • 高重合度キチンオリゴ糖の簡便な調製法
      28 Mar. 2005
    • キチングラフトポリアクリロニトリル加水分解物の合成とその特性解析
      26 Mar. 2005
    • N-アシルキトサン誘導体の特性解析
      26 Mar. 2005
    • Caulobacter crescentus CB15由来グルコアミラーゼの解析
      2005
    • Role of proline residues in conferring thermostability to aqualysin I
      Matsuzaki; M.; Sakaguchi; M.; Kawakita; M.
      3rd ISAT, 11 Nov. 2004, Kogakuin University
    • Substrate specificity of a serine protease aqualysin I from Thermus aquaticus YT-1 mutated at the substrate binding subsite S1
      Futagawa; Y.; Niimiya; K.; Sakaguchi; M.; Kawakita; M.
      3rd ISAT, 11 Nov. 2004, Kogakuin University
    • Analysis of functional roles of amino acid residues in glucoamylase from Clostridium sp.
      Sakaguchi; M.; Nankumo; T.; Mizukami; S.; Kuwabara; M. Kawakita; M.
      3rd ISAT, 11 Nov. 2004, Kogakuin University
    • Diurnal and occasional variation of excretion of diacetylspermine and creatinine in urine
      16 Oct. 2004
    • 細菌グルコアミラーゼの機能解析
      16 Sep. 2004
    • 細菌由来グルコアミラーゼの機能解析
      27 Mar. 2004
    • <i>Thermus aquaticus YT-1高度高熱性由来serine protease Aqualysin Iの高活性mutatntの構築
      27 Mar. 2004
    • Thermus aquaticus YT-1高度高熱性由来serine protease Aqualysin Iの高活性mutatntの構築
      2004
    • Substrate specificity of a serine protease aqualysin I from Thermus aquaticus YT-1 mutated at the substrate binding subsite S1
      2004
    • Role of proline residues in conferring thermostability to aqualysin I
      2004
    • Analysis of functional roles of amino acid residues in glucoamylase from Clostridium sp.
      2004
    • Substrate specificity of a serine protease aqualysin I from Thermus aquaticus YT-1 mutated at the substrate binding subsite S1
      3rd ISAT, 2004
    • Analysis of functional roles of amino acid residues in glucoamylase from Clostridium sp.
      3rd ISAT, 2004
    • The role of disulfide bond for activitiy and conformation in thermophilic alkaline serine protease, aqualysin I
      Sakaguchi; M.; Takezawa; M.; Nakazawa; R.; Kawakita; M.
      International Symposium on Advanced Technology(ISAT), 04 Nov. 2003, Southern Taiwan University of Science and Technology
    • The role of disulfide bond for activitiy and conformation in thermophilic alkaline serine protease, aqualysin I
      2003
    • 融合タンパク質を用いたアクアライシンIの大量発現系
      23 Sep. 1999
    • 有機合成反応におけるL-乳酸脱水素酵素の利用
      23 Sep. 1999
    • 油性酵素の合成とその特性解析
      23 Sep. 1999
    • グルコアミラーゼを用いたオリゴ糖合成反応の研究
      23 Sep. 1999
    • キトサンへのアクリルアミドのグラフト重合と生成物の凝集特性
      23 Sep. 1999
    • <i>Clostridium属由来グルコアミラーゼの反応機構の解析
      23 Sep. 1999
    • Clostridium属由来グルコアミラーゼの反応機構の解析
      1999
    • 細菌<i>Clostridium属由来グルコアミラーゼの大量発現系の確立
      15 Sep. 1998
    • 細菌<i>Clostridium属由来グルコアミラーゼの機能解析
      15 Sep. 1998
    • 細菌<i>Clostridium属由来グルコアミラーゼによる縮合反応の解析
      15 Sep. 1998
    • 細菌由来グルコアミラーゼの大量発現系の構築
      27 Mar. 1998
    • 酵素-脂質複合体による均一系酵素触媒重合
      27 Mar. 1998
    • 細菌由来グルコアミラーゼによるオリゴ糖の生成
      27 Mar. 1998
    • 細菌Clostridium属由来グルコアミラーゼの大量発現系の確立
      1998
    • 細菌Clostridium属由来グルコアミラーゼの機能解析
      1998
    • 細菌Clostridium属由来グルコアミラーゼによる縮合反応の解析
      1998

    Research Themes

    • Apr. 2017 - Mar. 2020
      Functional analysis of archaeal trehalases and attempt of their properties for novel oligosaccharides production
      Grant-in-Aid for Scientific Research (C), Kogakuin University
    • 2002 - 2012
      細菌由来新規グルコアミラーゼのクローニングと発現、及び特性解析
    • 2002 - 2012
      Cloning, expression, and characterization of novel glucoamylase from Caulobacter crescentus CB15
    • 1998 - 2012
      細菌グルコアミラーゼの機能と構造の解析
    • 1998 - 2012
      Analysis of function and structure in bacterial glucoamylase
    • 1999 - 2011
      好熱性タンパク質分解酵素の構造と機能の解析
    • 1999 - 2011
      Analysis of relationship between function and structure for thermophilic serine protease
    • 2011 - Present
      細菌キチナーゼの機能解析と改変
    • 2011 - Present
      Characterization and protein engineering for Bacterial chitinases